Academic Experts

Research AreaIndividual techniquesNameLast nameCountryOpen for academic collaborationOpen for cooperation with industryAvailable as paid serviceContactWeb link to more info
Computational
Ab Initio ModelingJakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Advanced Image AnalysisAlexeyBondarCzech Republicyesyesno
Advanced Image AnalysisDavideCalebiroUKyesyesyes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc)https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264
Constraint-based Stoichiometric ModelingEgilsStalidzansyesyesno
Contact Network AnalysisJakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Deciphering Off-Targets of GPCR ligandsKatarinaNikolicBulgariayesyesyes (deciphering off-targets of GPCR ligands)https://doi.org/10.1002/minf.201900165
Deep LearningSerbulenUnsalTurkeyyesyesyeshttps://www.biorxiv.org/content/10.1101/2020.10.28.359828v1
Density-Function Theory (DFT) Calculations of Reaction MechanismsAntoniosKolocourisGreeceyesyesno
Dynamic Pharmacophore Models (Dynophores)MarcelBermudezGermanyyesyesnohttps://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468
Dynamic Pharmacophore Models (Dynophores)GerhardWolberGermanyyesyesnohttps://doi.org/10.1002/wcms.1468
Free Energy CalculationAntoniosKolocourisGreeceyesyesno
Free Energy CalculationNicolasPanelSwedenyesnono
Free Energy CalculationJordiVillà-FreixaSpainyesyesyes (MD, free energy calculation of complex formation and allostery)https://github.com/CompBiochBiophLab/sbm-openmm
Homology ModelingMarcelBermudezGermanyyesyesnohttps://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468
Homology ModelingSławomirFilipekPolandyesyesno
Homology ModelingJakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Homology ModelingFabianLiessmannGermanyyesyesno
Homology ModelingMashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
In silico Drug DesignAnna MariaAlmericoItalyyesyesyes (in silico analysis and drug design)
In silico High-Throuput Screening (HTS)MarcelBermudezGermanyyesyesnohttps://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468
In silico High-Throuput Screening (HTS)FabianLiessmannGermanyyesyesno
In silico MutagenesisJakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Machine LearningMashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Machine LearningSerbulenUnsalTurkeyyesyesyeshttps://www.biorxiv.org/content/10.1101/2020.10.28.359828v1
Markov State ModelingJordiVillà-FreixaSpainyesyesyes (MD, free energy calculation of complex formation and allostery)https://github.com/CompBiochBiophLab/sbm-openmm
Mathematical ModelingJosefLazarCzech Republicyesyesnohttps://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf;
MetadynamicsBrianMedel Lacruzyesyesno
Molecular DockingGiuseppeDeganuttiUKyesyesno
Molecular DockingSławomirFilipekPolandyesyesno
Molecular DockingJakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Molecular DockingFabianLiessmannGermanyyesyesno
Molecular DockingMashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Molecular DockingNicolasPanelSwedenyesnono
Molecular Dynamics Simulations (MD)MarcelBermudezGermanyyesyesnohttps://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468
Molecular Dynamics Simulations (MD)GiuseppeDeganuttiUKyesyesno
Molecular Dynamics Simulations (MD)SławomirFilipekPolandyesyesno
Molecular Dynamics Simulations (MD)SalvatoreGuccioneItalyyesyesyes (Virtual Screening, Repositioning)
Molecular Dynamics Simulations (MD)JakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Molecular Dynamics Simulations (MD)AntoniosKolocourisGreeceyesyesno
Molecular Dynamics Simulations (MD)FabianLiessmannGermanyyesyesno
Molecular Dynamics Simulations (MD)MauroMaccarroneItalyyesyesno
Molecular Dynamics Simulations (MD)BrianMedel Lacruzyesyesno
Molecular Dynamics Simulations (MD)MashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Molecular Dynamics Simulations (MD)NicolasPanelSwedenyesnono
Molecular Dynamics Simulations (MD)JordiVillà-FreixaSpainyesyesyes (MD, free energy calculation of complex formation and allostery)https://github.com/CompBiochBiophLab/sbm-openmm
Molecular Dynamics Simulations (MD)GerhardWolberGermanyyesyesnohttps://doi.org/10.1002/wcms.1468
Molecular ModelingBeatrizBueschbellPortugalyesyesno
Molecular ModelingEsterKellenbergerFranceyesyesno
Molecular ModelingXavierRoviraSpainyesyesyes (Drug design)https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037
Non Equilibrium Molecular Dynamics (MD) SimulationGiuseppeDeganuttiUKyesyesno
Ordinary Differential Equations (ODE)EgilsStalidzansyesyesno
Protein-Protein DockingFabianLiessmannGermanyyesyesno
Protein Representation LearningSerbulenUnsalTurkeyyesyesyeshttps://www.biorxiv.org/content/10.1101/2020.10.28.359828v1
Receptor ModelingGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Replica Exchange Molecular Dynamics with Solute Tempering (REST2)JakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Steered Molecular Dynamics (SMD)SławomirFilipekPolandyesyesno
Structure-based Model SimulationsJordiVillà-FreixaSpainyesyesyes (MD, free energy calculation of complex formation and allostery)https://github.com/CompBiochBiophLab/sbm-openmm
Supervised Molecular Dynamics (SuMD)SławomirFilipekPolandyesyesno
Supervised Molecular Dynamics (SuMD)JakubJakowieckiPolandyesyesnoSuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499
Ultra-Large Library ScreeningFabianLiessmannGermanyyesyesno
Virtual ScreeningSalvatoreGuccioneItalyyesyesyes (Virtual Screening, Repositioning)
Virtual ScreeningEsterKellenbergerFranceyesyesno
Virtual ScreeningGerhardWolberGermanyyesyesnohttps://doi.org/10.1002/wcms.1468
Bioinformatics
Kinetic models of ligand binding and receptor activationAgo RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Next generation sequencing (NGS) AnalysisSerbulenUnsalTurkeyyesyesyeshttps://www.biorxiv.org/content/10.1101/2020.10.28.359828v1
Protein Function PredictionSerbulenUnsalTurkeyyesyesyeshttps://www.biorxiv.org/content/10.1101/2020.10.28.359828v1
Statistical ModelingSerbulenUnsalTurkeyyesyesyeshttps://www.biorxiv.org/content/10.1101/2020.10.28.359828v1
Structure-based energy calculations of Protein-Protein Interactions (PPIs)MickeyKosloffIsraelyesyesnohttp://koslofflab.haifa.ac.il/papers.html
Structural BioinformaticsMickeyKosloffIsraelyesyesnohttp://koslofflab.haifa.ac.il/papers.html
Structural
CryoElectron Microscopy (CryoEM)GebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
CrystallizationMartinCaffreyIrelandyesyesnohttps://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using
Dynamic Nuclear Magnetic Resonance (NMR) SpectroscopyAntoniosKolocourisGreeceyesyesno
Free Electron LasersGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Kinetic CrystallographyGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Lipid Cubic Phase CrystallizationMartinCaffreyIrelandyesyesnohttps://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using
Mass SpectrometryJuan BautistaBlanco-CanosaSpainyesyesno
Nuclear Magnetic Resonance (NMR)FrankBernhardGermanyyesyesno
Nuclear Magnetic Resonance (NMR)MarkusMuttenthalerAustriayesyesyes (Custom peptide synthesis and probe development)
Room Temperature Serial CrystallographyGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Small-angle X-ray Scattering (SAXS)AntoniosKolocourisGreeceyesyesno
Small-angle X-ray Scattering (SAXS)MauroMaccarroneItalyyesyesno
Solid State Nuclear Magnetic Resonance (NMR) SpectroscopyAntoniosKolocourisGreeceyesyesno
Solution Nuclear Magnetic Resonance (NMR) SpectroscopyAntoniosKolocourisGreeceyesyesno
Structural Analysis of Drug-Receptor InteractionsAnna MariaAlmericoItalyyesyesyes (in silico analysis and drug design)
Structure AnalysisMickeyKosloffIsraelyesyesnohttp://koslofflab.haifa.ac.il/papers.html
Wide-angle X-ray Scattering (WAXS)AntoniosKolocourisGreeceyesyesno
X-ray crystallographyMartinCaffreyIrelandyesyesnohttps://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using
X-ray crystallographyAntoniosKolocourisGreeceyesyesno
X-ray crystallographyJosefLazarCzech Republicyesyesnohttps://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf;
X-ray crystallographyGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Biophysical
Automated Electrophysiology Two Electrode-Voltage-Clamp (TEVC)ChristopheMoreauFranceyesyesno
Bioluminescence Resonance Energy Transfer (BRET)AndyChevignéLuxembourgyesyesyes (Drug screening, molecular profiling)
Bioluminescence Resonance Energy Transfer (BRET)RafaelFrancoyesyesno
Bioluminescence Resonance Energy Transfer (BRET)Raphael SilvanusHaiderGermanyyesnono
Bioluminescence Resonance Energy Transfer (BRET)JulienHansonBelgiumyesyesno
Bystander Bioluminescence Resonance Energy Transfer (BRET)ShannonO'BrienUKyesyesno
Confocal MicroscopyRafaelFrancoyesyesno
Confocal MicroscopyRaphael SilvanusHaiderGermanyyesnono
Confocal MicroscopyJulienHansonBelgiumyesyesno
Confocal MicroscopySilviaSposiniFranceyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Differential Scanning Calorimetry (DSC)AntoniosKolocourisGreeceyesyesno
Fiber Photometry in vivo ImagingTommasoPatriarchiSwitzerlandyesnonohttps://www.patriarchilab.com/
Flow ImagingAndyChevignéLuxembourgyesyesyes (Drug screening, molecular profiling)
Fluorescence Anisotropy (Ligand Binding Assay)IsabelAlvesFranceyesyesnohttps://pubs.acs.org/doi/full/10.1021/acs.accounts.9b00007
Fluorescence Anisotropy (Ligand Binding Assay)Ago RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Fluorescence Lifetime Imaging (FLIM)Ago RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Fluorescence MicroscopyBeatrizBueschbellPortugalyesyesno
Fluorescence MicroscopyJosefLazarCzech Republicyesyesnohttps://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf;
Fluorescence MicroscopyAgo RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Fluorescence SpectroscopyMarijaJankunecLithuaniayesyesyes (high-speed AFM, lipid-protein interaction)https://www.ribm.co.jp/en/product/sub_ssnex_en
Force SpectroscopyMarijaJankunecLithuaniayesyesyes (high-speed AFM, lipid-protein interaction)https://www.ribm.co.jp/en/product/sub_ssnex_en
Forster Resonance Energy Transfer (FRET)KjetilAndressenNorwayyesyesno
Forster Resonance Energy Transfer (FRET)DavideCalebiroUKyesyesyes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc)https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264
Forster Resonance Energy Transfer (FRET)RafaelFrancoyesyesno
Forster Resonance Energy Transfer (FRET)JulienHansonBelgiumyesyesno
Forster Resonance Energy Transfer (FRET)XavierRoviraSpainyesyesyes (Drug design)https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037
High-Speed Atomic Force MicroscopyMarijaJankunecLithuaniayesyesyes (high-speed AFM, lipid-protein interaction)https://www.ribm.co.jp/en/product/sub_ssnex_en
Highly Inclined Thin Illumination (HILO) ImagingShannonO'BrienUKyesyesno
Imaging LigandsHolgerStarkGermanyyesyesyes (binding assays dopamine and histamine receptor subtypes; MAO A/B screening; ligand synthesis)
Ligand Binding at Single-Molecule LevelAgo RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Microelectrode RecordingBelginBuyukakilliTurkeyyesnono
Multicell Forster Resonance Energy Transfer (FRET)KjetilAndressenNorwayyesyesno
NanoBITAndyChevignéLuxembourgyesyesyes (Drug screening, molecular profiling)
NanoBITShannonO'BrienUKyesyesno
Nano Bioluminescence Resonance Energy Transfer (NanoBRET)AndyChevignéLuxembourgyesyesyes (Drug screening, molecular profiling)
Nano Bioluminescence Resonance Energy Transfer (NanoBRET)ShannonO'BrienUKyesyesno
Plasmon Waveguide ResonanceIsabelAlvesFranceyesyesnohttps://pubs.acs.org/doi/full/10.1021/acs.accounts.9b00007
Polarization-resolved Fluorescence MicroscopyJosefLazarCzech Republicyesyesnohttps://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf;
Protein-Membrane InteractionsAntoniosKolocourisGreeceyesyesno
Pulse pH AssaySilviaSposiniFranceyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Single Cell Fluorescence AssaysKarenMartinezDenmarkyesyesno
Single-Molecule Microscopy (SMM)AlexeyBondarCzech Republicyesyesno
Single-Molecule Microscopy (SMM)DavideCalebiroUKyesyesyes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc)https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264
SpectroscopyGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Superresolution MicroscopyDavideCalebiroUKyesyesyes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc)https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264
Superresolution MicroscopySilviaSposiniFranceyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Thermo Bioluminescence Resonance Energy Transfer (ThermoBRET)DavidSykesUKyesyesyes (thermostability based drug screening)https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1
Thermo Forster Resonance Energy Transfer (ThermoFRET)DavidSykesUKyesyesyes (thermostability based drug screening)https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1
Time-resolved Forster Resonance Energy Transfer (Time-resolved FRET)RafaelFrancoyesyesno
Total Internal Reflection Fluorescence Microscopy (TIRF)AlexeyBondarCzech Republicyesyesno
Total Internal Reflection Fluorescence Microscopy (TIRF)DavideCalebiroUKyesyesyes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc)https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264
Total Internal Reflection Fluorescence Microscopy (TIRF)SilviaSposiniFranceyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Total Internal Reflection Fluorescence Microscopy (TIRF)Ago RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Biochemical
Cell-free Expression of Membrane ProteinsFrankBernhardGermanyyesyesnoRues et al (2016) BBA 1858, 1306; Rues et al (2018) Methods 147, 73; Dong et al (2018) JMB 430, 5105; Krug et al (2020) Angew Chemie 59, 2.
ChromatographyJoséZugazaSpainyesyesno
Fluorescence Size Exclusion ChromatographyGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Genetically-Encoded Fluorescent GPCR SensorsTommasoPatriarchiSwitzerlandyesnonohttps://www.patriarchilab.com/
ImmunofluorescenceAndrejaAmbriović-RistovCroatiayesyesno
In vitro enzymatic reactionsJoséZugazaSpainyesyesno
Isolation of Integrin Adhesion ComplexesAndrejaAmbriović-RistovCroatiayesyesno
Label-Free technologiesRafaelFrancoyesyesno
Ligand Binding AssaysAgo RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
Ligand Binding AssaysGebhardSchertlerSwitzerlandyesyesyes (Electron Microscopy; GPCR Structure; Crystallography)https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research
Ligand Binding AssaysDavidSykesUKyesyesyes (thermostability based drug screening)https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1
Protein Chemical LigationJuan BautistaBlanco-CanosaSpainyesyesno
Protein Production and PurificationMartinCaffreyIrelandyesyesnohttps://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using
Recombinant PeptidesJoséZugazaSpainyesyesno
Solid Phase Peptide Synthesis (SPPS)Juan BautistaBlanco-CanosaSpainyesyesno
Thermostability AssaysDavidSykesUKyesyesyes (thermostability based drug screening)https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1
ThermophoresisIsabelAlvesFranceyesyesnohttps://pubs.acs.org/doi/full/10.1021/acs.accounts.9b00007
Cell biology
Apoptosis AssaysNunoSaraivaPortugalyesnono
Cell-based AssaysBeatrizBueschbellPortugalyesyesno
Cell Motility (Invasion/Migration; 2D/3D)NunoSaraivaPortugalyesnono
CRISPR/Cas9 Cell LinesJulienHansonBelgiumyesyesno
ElectrophysiologyAntoniosKolocourisGreeceyesyesno
GPCR heterologous expression and characterization in Xenopus oocytesChristopheMoreauFranceyesyesno
GPCR internalizationShannaBastiaan-NetNetherlandsyesyesyes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation)
IncucyteMarkusMuttenthalerAustriayesyesyes (Custom peptide synthesis and probe development)
Insect cell cultureAndyChevignéLuxembourgyesyesyes (Drug screening, molecular profiling)
Mitochondria AssaysSilvanaAndricSerbiayesyesyes (In vivo models (rat, mice); molecular biology and functional assays of endocrine cells)
Reporter Cell LinesShannaBastiaan-NetNetherlandsyesyesyes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation)
Molecular biology
CRISPR/Cas9JulienHansonBelgiumyesyesno
CRISPR/Cas10JosefLazarCzech Republicyesyesnohttps://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf;
IP-One AssayMashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Mutagenesis, Chimeras, PhusionsSilvanaAndricSerbiayesyesyes (In vivo models (rat, mice); molecular biology and functional assays of endocrine cells)
Mutagenesis, Chimeras, PhusionsRafaelFrancoyesyesno
Mutagenesis, Chimeras, PhusionsJulienHansonBelgiumyesyesno
Mutagenesis, Chimeras, PhusionsChristopheMoreauFranceyesyesno
PyrosequencingMauroMaccarroneItalyyesyesno
qPCRShannaBastiaan-NetNetherlandsyesyesyes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation)
Receptors in BaculovirusesAgo RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
RNAseqShannaBastiaan-NetNetherlandsyesyesyes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation)
Pharmacology
Ca 2+ Concentration AssaysShannaBastiaan-NetNetherlandsyesyesyes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation)
Ca 2+ Concentration AssaysJulienHansonBelgiumyesyesno
cAMP Concentration AssaysRafaelFrancoyesyesno
cAMP Concentration AssaysJulienHansonBelgiumyesyesno
cAMP Concentration AssaysAgo RinkenEstoniayesyesyes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF)DOI: 10.1039/d0nr06737g
ERK PhoshorylationRafaelFrancoyesyesno
ERK PhoshorylationJulienHansonBelgiumyesyesno
Functional Analysis of Endocriine CellsSilvanaAndricSerbiayesyesyes (In vivo models (rat, mice); molecular biology and functional assays of endocrine cells)
GTPase AssaysMickeyKosloffIsraelyesyesnohttp://koslofflab.haifa.ac.il/papers.html
Intracellular ROS detectionNunoSaraivaPortugalyesnono
NanoLuc ComplementationJulienHansonBelgiumyesyesno
Photopharmacological assaysXavierRoviraSpainyesyesyes (Drug design)https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037
Protein-Protein Interactions (PPIs) AssaysMickeyKosloffIsraelyesyesnohttp://koslofflab.haifa.ac.il/papers.html
RhoGEF ActivityJulienHansonBelgiumyesyesno
TGFalpha SheddingJulienHansonBelgiumyesyesno
Chemistry
Combinatorial Synthesis (Conjugation and Bioconjugation)MariaMajellaroSpainyesyesyes (on-demand synthesis)https://www.celtarys.com
Enantioselective Synthesis/Chiral HPLCEddySoteloSpainyesyesyes (custom synthesis and optimization of fluorescent ligands)
Enantioselective Synthesis/Chiral HPLCMariaMajellaroSpainyesyesyes (on-demand synthesis)https://www.celtarys.com
Fluorescent Ligand DevelopmentMariaMajellaroSpainyesyesyes (on-demand synthesis)https://www.celtarys.com
Fluorescent Ligand DevelopmentEddySoteloSpainyesyesyes (custom synthesis and optimization of fluorescent ligands)
High-throughput screening of fluorescent ligandsEddySoteloSpainyesyesyes (custom synthesis and optimization of fluorescent ligands)
Hit to Lead OptimizationMarcelBermudezGermanyyesyesnohttps://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468
Hit to Lead OptimizationMariaMajellaroSpainyesyesyes (on-demand synthesis)https://www.celtarys.com
Ligand-Based Drug DesignKatarinaNikolicBulgariayesyesyes (deciphering off-targets of GPCR ligands)https://doi.org/10.1002/minf.201900165
Medicinal ChemistryEsterKellenbergerFranceyesyesno
Medicinal ChemistryAntoniosKolocourisGreeceyesyesno
Medicinal ChemistryMarkusMuttenthalerAustriayesyesyes (Custom peptide synthesis and probe development)
Medicinal ChemistryHolgerStarkGermanyyesyesyes (binding assays dopamine and histamine receptor subtypes; MAO A/B screening; ligand synthesis)
Multivalent Ligand DesignMarkusMuttenthalerAustriayesyesyes (Custom peptide synthesis and probe development)
NanoparticlesFrankBernhardGermanyyesyesnoRues et al (2016) BBA 1858, 1306; Rues et al (2018) Methods 147, 73; Dong et al (2018) JMB 430, 5105; Krug et al (2020) Angew Chemie 59, 2.
Nuclear Magnetic Resonance (NMR) for compounds characterizationEddySoteloSpainyesyesyes (custom synthesis and optimization of fluorescent ligands)
Organic Compound Purification and CharacterizationCristinaNativiItalyyesyesno
Organic SynthesisSalvatoreGuccioneItalyyesyesyes (Virtual Screening, Repositioning)
Organic SynthesisCristinaNativiItalyyesyesno
Structure-based Drug DevelopmentAnna MariaAlmericoItalyyesyesyes (in silico analysis and drug design)
Structure-based Drug DevelopmentFabianLiessmannGermanyyesyesno
Structure-based Drug DevelopmentKatarinaNikolicBulgariayesyesyes (deciphering off-targets of GPCR ligands)https://doi.org/10.1002/minf.201900165
Structure-based Drug DevelopmentXavierRoviraSpainyesyesyes (Drug design)https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037
Structure-based Drug DevelopmentHolgerStarkGermanyyesyesyes (binding assays dopamine and histamine receptor subtypes; MAO A/B screening; ligand synthesis)
Synthesis of drugs with heterocyclic moietiesAnna MariaAlmericoItalyyesyesyes (in silico analysis and drug design)
Tracer DevelopmentMarkusMuttenthalerAustriayesyesyes (Custom peptide synthesis and probe development)
Animal models
CRISPR/Cas9 MiceJulienHansonBelgiumyesyesno
Heart Failure with Preserved Ejection Fraction (HFpEF) Animal ModelKjetilAndressenNorwayyesyesno
In vivo Imaging (Fibre Photometry)TommasoPatriarchiSwitzerlandyesnonohttps://www.patriarchilab.com/
Zebrafish Behhavioral AssaysXavierRoviraSpainyesyesyes (Drug design)https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037
Behavioral
Online SurveysMashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Sensory Evaluation in Human Panels (Taste Related)MashaNivIsraelyesyesnohttps://pubmed.ncbi.nlm.nih.gov/32807908/
Clinical
Electrocardiography (ECG)BelginBuyukakilliTurkeyyesnono
Electromyopraphy (EMG)BelginBuyukakilliTurkeyyesnono
Electroneurography (ENG)BelginBuyukakilliTurkeyyesnono
Impedance Cardiography (ICG-TEB)BelginBuyukakilliTurkeyyesnono

COST

COST (European Cooperation in Science and Technology) is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation.

COST Action CA18133

All cells face the vital challenge of sensing their environments and responding in appropriate ways. How are different signalling pathways activated and modulated in precise and reproducible ways? Filling this gap in knowledge is absolutely necessary to advance the next generation of pharmaceutical drugs.

COST Action

COST Action

Pulpit rock

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