Computational | | | | | | | | | |
| Ab Initio Modeling | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Advanced Image Analysis | Alexey | Bondar | Czech Republic | yes | yes | no | | |
| Advanced Image Analysis | Davide | Calebiro | UK | yes | yes | yes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc) | | https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264 |
| Binding Energy Calculation | Juan | Fernandez-Recio | Spain | yes | yes | yes (Structural modeling of protein-protein interactions by docking, and energetic impact of mutations on protein complexes) | | https://life.bsc.es/pid/pidweb/default/tools |
| Chemoinformatics Analysis | Antonella | Di Pizio | Germany | yes | yes | no | | |
| Chemoinformatics Analysis | Irina | Tikhonova | UK | yes | yes | yes (molecule design) | | |
| Constraint-based Stoichiometric Modeling | Egils | Stalidzans | | yes | yes | no | | |
| Contact Network Analysis | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Deciphering Off-Targets of GPCR ligands | Katarina | Nikolic | Bulgaria | yes | yes | yes (deciphering off-targets of GPCR ligands) | | https://doi.org/10.1002/minf.201900165 |
| Deep Learning | Serbulent | Unsal | Turkey | yes | yes | yes | | https://www.biorxiv.org/content/10.1101/2020.10.28.359828v1 |
| Density-Function Theory (DFT) Calculations of Reaction Mechanisms | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Dynamic Pharmacophore Models (Dynophores) | Marcel | Bermudez | Germany | yes | yes | no | | https://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468 |
| Dynamic Pharmacophore Models (Dynophores) | Gerhard | Wolber | Germany | yes | yes | no | | https://doi.org/10.1002/wcms.1468 |
| Dynamic Time Warping | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Free Energy Calculation | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Free Energy Calculation | Nicolas | Panel | Sweden | yes | no | no | | |
| Free Energy Calculation | Jordi | Villà-Freixa | Spain | yes | yes | yes (MD, free energy calculation of complex formation and allostery) | | https://github.com/CompBiochBiophLab/sbm-openmm |
| GUI development | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Homology Modeling | Marcel | Bermudez | Germany | yes | yes | no | | https://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468 |
| Homology Modeling | Sławomir | Filipek | Poland | yes | yes | no | | |
| Homology Modeling | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Homology Modeling | Fabian | Liessmann | Germany | yes | yes | no | | |
| Homology Modeling | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Hydrogen-bond Networks (Graph-based Analysis) | Ana-Nicoleta | Bondar | Romania | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/31652399/, https://pubmed.ncbi.nlm.nih.gov/33007367/ |
| In silico Drug Design | Anna Maria | Almerico | Italy | yes | yes | yes (in silico analysis and drug design) | | |
| In silico High-Throuput Screening (HTS) | Marcel | Bermudez | Germany | yes | yes | no | | https://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468 |
| In silico High-Throuput Screening (HTS) | Fabian | Liessmann | Germany | yes | yes | no | | |
| In silico Mutagenesis | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Interface Prediction | Juan | Fernandez-Recio | Spain | yes | yes | yes (Structural modeling of protein-protein interactions by docking, and energetic impact of mutations on protein complexes) | | https://life.bsc.es/pid/pidweb/default/tools |
| Machine Learning | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Machine Learning | Serbulent | Unsal | Turkey | yes | yes | yes | | https://www.biorxiv.org/content/10.1101/2020.10.28.359828v1 |
| Markov State Modeling | Jordi | Villà-Freixa | Spain | yes | yes | yes (MD, free energy calculation of complex formation and allostery) | | https://github.com/CompBiochBiophLab/sbm-openmm |
| Mathematical Modeling | Josef | Lazar | Czech Republic | yes | yes | no | | https://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf; |
| Metadynamics | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Metadynamics | Brian | Medel Lacruz | | yes | yes | no | | |
| Molecular Docking | Giuseppe | Deganutti | UK | yes | yes | no | | |
| Molecular Docking | Sławomir | Filipek | Poland | yes | yes | no | | |
| Molecular Docking | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Molecular Docking | Fabian | Liessmann | Germany | yes | yes | no | | |
| Molecular Docking | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Molecular Docking | Nicolas | Panel | Sweden | yes | no | no | | |
| Molecular Docking | Agata | Zieba | Poland | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Marcel | Bermudez | Germany | yes | yes | no | | https://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468 |
| Molecular Dynamics Simulations (MD) | Giuseppe | Deganutti | UK | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Antonella | Di Pizio | Germany | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Sławomir | Filipek | Poland | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) Large scale | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Molecular Dynamics Simulations (MD) | Salvatore | Guccione | Italy | yes | yes | yes (Virtual Screening, Repositioning) | | |
| Molecular Dynamics Simulations (MD) | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Molecular Dynamics Simulations (MD) | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Fabian | Liessmann | Germany | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Mauro | Maccarrone | Italy | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Brian | Medel Lacruz | | yes | yes | no | | |
| Molecular Dynamics Simulations (MD) | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Molecular Dynamics Simulations (MD) | Nicolas | Panel | Sweden | yes | no | no | | |
| Molecular Dynamics Simulations (MD) | Irina | Tikhonova | UK | yes | yes | yes (molecule design) | | |
| Molecular Dynamics Simulations (MD) | Jordi | Villà-Freixa | Spain | yes | yes | yes (MD, free energy calculation of complex formation and allostery) | | https://github.com/CompBiochBiophLab/sbm-openmm |
| Molecular Dynamics Simulations (MD) | Gerhard | Wolber | Germany | yes | yes | no | | https://doi.org/10.1002/wcms.1468 |
| Molecular Modeling | Beatriz | Bueschbell | Portugal | yes | yes | no | | |
| Molecular Modeling | Ester | Kellenberger | France | yes | yes | no | | |
| Molecular Modeling | Xavier | Rovira | Spain | yes | yes | yes (Drug design) | | https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037 |
| Non Equilibrium Molecular Dynamics (MD) Simulation | Giuseppe | Deganutti | UK | yes | yes | no | | |
| Ordinary Differential Equations (ODE) | Egils | Stalidzans | | yes | yes | no | | |
| Protein-Protein Docking | Juan | Fernandez-Recio | Spain | yes | yes | yes (Structural modeling of protein-protein interactions by docking, and energetic impact of mutations on protein complexes) | | https://life.bsc.es/pid/pidweb/default/tools |
| Protein-Protein Docking | Fabian | Liessmann | Germany | yes | yes | no | | |
| Protein Representation Learning | Serbulent | Unsal | Turkey | yes | yes | yes | | https://www.biorxiv.org/content/10.1101/2020.10.28.359828v1 |
| Quantitative Structure-Activity Relationship (QSAR) | Agata | Zieba | Poland | yes | yes | no | | |
| Receptor Modeling | Ana-Nicoleta | Bondar | Romania | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/31652399/, https://pubmed.ncbi.nlm.nih.gov/33007367/ |
| Receptor Modeling | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Replica Exchange Molecular Dynamics with Solute Tempering (REST2) | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Steered Molecular Dynamics (SMD) | Sławomir | Filipek | Poland | yes | yes | no | | |
| Structure-based Model Simulations | Jordi | Villà-Freixa | Spain | yes | yes | yes (MD, free energy calculation of complex formation and allostery) | | https://github.com/CompBiochBiophLab/sbm-openmm |
| Supervised Molecular Dynamics (SuMD) | Sławomir | Filipek | Poland | yes | yes | no | | |
| Supervised Molecular Dynamics (SuMD) | Jakub | Jakowiecki | Poland | yes | yes | no | | SuMD: 10.3390/molecules25081930 10.1021/acs.jcim.6b00499 |
| Ultra-Large Library Screening | Fabian | Liessmann | Germany | yes | yes | no | | |
| Virtual Screening | Antonella | Di Pizio | Germany | yes | yes | no | | |
| Virtual Screening | Salvatore | Guccione | Italy | yes | yes | yes (Virtual Screening, Repositioning) | | |
| Virtual Screening | Ester | Kellenberger | France | yes | yes | no | | |
| Virtual Screening | Irina | Tikhonova | UK | yes | yes | yes (molecule design) | | |
| Virtual Screening | Gerhard | Wolber | Germany | yes | yes | no | | https://doi.org/10.1002/wcms.1468 |
| | | | | | | | | |
Bioinformatics | | | | | | | | | |
| Deep Learning-based Sequence Analysis | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Kinetic models of ligand binding and receptor activation | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| Next generation sequencing (NGS) Analysis | Serbulent | Unsal | Turkey | yes | yes | yes | | https://www.biorxiv.org/content/10.1101/2020.10.28.359828v1 |
| Protein Function Prediction | Serbulent | Unsal | Turkey | yes | yes | yes | | https://www.biorxiv.org/content/10.1101/2020.10.28.359828v1 |
| Regression models | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Statistical Modeling | Serbulent | Unsal | Turkey | yes | yes | yes | | https://www.biorxiv.org/content/10.1101/2020.10.28.359828v1 |
| Structure-based energy calculations of Protein-Protein Interactions (PPIs) | Mickey | Kosloff | Israel | yes | yes | no | | http://koslofflab.haifa.ac.il/papers.html |
| Structural Bioinformatics | Mickey | Kosloff | Israel | yes | yes | no | | http://koslofflab.haifa.ac.il/papers.html |
Structural | | | | | | | | | |
| CryoElectron Microscopy (CryoEM) | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Crystallization | Martin | Caffrey | Ireland | yes | yes | no | | https://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using |
| Dynamic Nuclear Magnetic Resonance (NMR) Spectroscopy | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Free Electron Lasers | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Kinetic Crystallography | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Lipid Cubic Phase Crystallization | Martin | Caffrey | Ireland | yes | yes | no | | https://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using |
| Mass Spectrometry | Juan Bautista | Blanco-Canosa | Spain | yes | yes | no | | |
| Nuclear Magnetic Resonance (NMR) | Frank | Bernhard | Germany | yes | yes | no | | |
| Nuclear Magnetic Resonance (NMR) | Markus | Muttenthaler | Austria | yes | yes | yes (Custom peptide synthesis and probe development) | | |
| Room Temperature Serial Crystallography | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Small-angle X-ray Scattering (SAXS) | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Small-angle X-ray Scattering (SAXS) | Mauro | Maccarrone | Italy | yes | yes | no | | |
| Solid State Nuclear Magnetic Resonance (NMR) Spectroscopy | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Solution Nuclear Magnetic Resonance (NMR) Spectroscopy | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Structural Analysis of Drug-Receptor Interactions | Anna Maria | Almerico | Italy | yes | yes | yes (in silico analysis and drug design) | | |
| Structure Analysis | Mickey | Kosloff | Israel | yes | yes | no | | http://koslofflab.haifa.ac.il/papers.html |
| Structure Assessment and Preparation, Automation | Toni | Giorgino | Italy | yes | yes | yes (Assessment on in silico feasibility, Large scale MD, Modeling) | | https://dynamictimewarping.github.io/ |
| Wide-angle X-ray Scattering (WAXS) | Antonios | Kolocouris | Greece | yes | yes | no | | |
| X-ray crystallography | Martin | Caffrey | Ireland | yes | yes | no | | https://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using |
| X-ray crystallography | Antonios | Kolocouris | Greece | yes | yes | no | | |
| X-ray crystallography | Josef | Lazar | Czech Republic | yes | yes | no | | https://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf; |
| X-ray crystallography | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| | | | | | | | | |
Biophysical | | | | | | | | | |
| Automated Electrophysiology Two Electrode-Voltage-Clamp (TEVC) | Christophe | Moreau | France | yes | yes | no | | |
| Bioluminescence Resonance Energy Transfer (BRET) | Andy | Chevigné | Luxembourg | yes | yes | yes (Drug screening, molecular profiling) | | |
| Bioluminescence Resonance Energy Transfer (BRET) | Rafael | Franco | | yes | yes | no | | |
| Bioluminescence Resonance Energy Transfer (BRET) | Raphael Silvanus | Haider | Germany | yes | no | no | | |
| Bioluminescence Resonance Energy Transfer (BRET) | Julien | Hanson | Belgium | yes | yes | no | | |
| Bioluminescence Resonance Energy Transfer (uBRET) | Xavier | Leroy | | yes | no | yes (Biosens-all BRET) | | https://www.domaintherapeutics.com/technologies/ |
| Bystander Bioluminescence Resonance Energy Transfer (BRET) | Shannon | O'Brien | UK | yes | yes | no | | |
| Confocal Microscopy | Rafael | Franco | | yes | yes | no | | |
| Confocal Microscopy | Raphael Silvanus | Haider | Germany | yes | no | no | | |
| Confocal Microscopy | Julien | Hanson | Belgium | yes | yes | no | | |
| Confocal Microscopy | Silvia | Sposini | France | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Differential Scanning Calorimetry (DSC) | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Fiber Photometry in vivo Imaging | Tommaso | Patriarchi | Switzerland | yes | no | no | | https://www.patriarchilab.com/ |
| Flow Imaging | Andy | Chevigné | Luxembourg | yes | yes | yes (Drug screening, molecular profiling) | | |
| Fluorescence Anisotropy (Ligand Binding Assay) | Isabel | Alves | France | yes | yes | no | | https://pubs.acs.org/doi/full/10.1021/acs.accounts.9b00007 |
| Fluorescence Anisotropy (Ligand Binding Assay) | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| Fluorescence Lifetime Imaging (FLIM) | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| Fluorescence Microscopy | Beatriz | Bueschbell | Portugal | yes | yes | no | | |
| Fluorescence Microscopy | Josef | Lazar | Czech Republic | yes | yes | no | | https://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf; |
| Fluorescence Microscopy | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| Fluorescence Spectroscopy | Marija | Jankunec | Lithuania | yes | yes | yes (high-speed AFM, lipid-protein interaction) | | https://www.ribm.co.jp/en/product/sub_ssnex_en |
| Force Spectroscopy | Marija | Jankunec | Lithuania | yes | yes | yes (high-speed AFM, lipid-protein interaction) | | https://www.ribm.co.jp/en/product/sub_ssnex_en |
| Forster Resonance Energy Transfer (FRET) | Kjetil | Andressen | Norway | yes | yes | no | | |
| Forster Resonance Energy Transfer (FRET) | Davide | Calebiro | UK | yes | yes | yes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc) | | https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264 |
| Forster Resonance Energy Transfer (FRET) | Rafael | Franco | | yes | yes | no | | |
| Forster Resonance Energy Transfer (FRET) | Julien | Hanson | Belgium | yes | yes | no | | |
| Forster Resonance Energy Transfer (FRET) | Xavier | Rovira | Spain | yes | yes | yes (Drug design) | | https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037 |
| High-Speed Atomic Force Microscopy | Marija | Jankunec | Lithuania | yes | yes | yes (high-speed AFM, lipid-protein interaction) | | https://www.ribm.co.jp/en/product/sub_ssnex_en |
| Highly Inclined Thin Illumination (HILO) Imaging | Shannon | O'Brien | UK | yes | yes | no | | |
| Imaging Ligands | Holger | Stark | Germany | yes | yes | yes (binding assays dopamine and histamine receptor subtypes; MAO A/B screening; ligand synthesis) | | |
| Ligand Binding at Single-Molecule Level | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| Microelectrode Recording | Belgin | Buyukakilli | Turkey | yes | no | no | | |
| Multicell Forster Resonance Energy Transfer (FRET) | Kjetil | Andressen | Norway | yes | yes | no | | |
| NanoBIT | Andy | Chevigné | Luxembourg | yes | yes | yes (Drug screening, molecular profiling) | | |
| NanoBIT | Shannon | O'Brien | UK | yes | yes | no | | |
| Nano Bioluminescence Resonance Energy Transfer (NanoBRET) | Andy | Chevigné | Luxembourg | yes | yes | yes (Drug screening, molecular profiling) | | |
| Nano Bioluminescence Resonance Energy Transfer (NanoBRET) | Shannon | O'Brien | UK | yes | yes | no | | |
| Plasmon Waveguide Resonance | Isabel | Alves | France | yes | yes | no | | https://pubs.acs.org/doi/full/10.1021/acs.accounts.9b00007 |
| Polarization-resolved Fluorescence Microscopy | Josef | Lazar | Czech Republic | yes | yes | no | | https://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf; |
| Protein-Membrane Interactions | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Pulse pH Assay | Silvia | Sposini | France | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Single Cell Fluorescence Assays | Karen | Martinez | Denmark | yes | yes | no | | |
| Single-Molecule Microscopy (SMM) | Alexey | Bondar | Czech Republic | yes | yes | no | | |
| Single-Molecule Microscopy (SMM) | Davide | Calebiro | UK | yes | yes | yes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc) | | https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264 |
| Spectroscopy | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Superresolution Microscopy | Davide | Calebiro | UK | yes | yes | yes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc) | | https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264 |
| Superresolution Microscopy | Silvia | Sposini | France | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Thermo Bioluminescence Resonance Energy Transfer (ThermoBRET) | David | Sykes | UK | yes | yes | yes (thermostability based drug screening) | | https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1 |
| Thermo Forster Resonance Energy Transfer (ThermoFRET) | David | Sykes | UK | yes | yes | yes (thermostability based drug screening) | | https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1 |
| Time-resolved Forster Resonance Energy Transfer (Time-resolved FRET) | Rafael | Franco | | yes | yes | no | | |
| Total Internal Reflection Fluorescence Microscopy (TIRF) | Alexey | Bondar | Czech Republic | yes | yes | no | | |
| Total Internal Reflection Fluorescence Microscopy (TIRF) | Davide | Calebiro | UK | yes | yes | yes (advanced microscopy and image analysis techniques (FRET, single molecule, superresolution, lattice light sheet, multiphoton, etc) | | https://www.birmingham.ac.uk/university/colleges/mds/facilities/compare/compare.aspx; https://www.nature.com/articles/nature24264 |
| Total Internal Reflection Fluorescence Microscopy (TIRF) | Silvia | Sposini | France | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Total Internal Reflection Fluorescence Microscopy (TIRF) | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
Biochemical | | | | | | | | | |
| Cell-free Expression of Membrane Proteins | Frank | Bernhard | Germany | yes | yes | no | | Rues et al (2016) BBA 1858, 1306; Rues et al (2018) Methods 147, 73; Dong et al (2018) JMB 430, 5105; Krug et al (2020) Angew Chemie 59, 2. |
| Chromatography | José | Zugaza | Spain | yes | yes | no | | |
| Drug Discovery | Xavier | Leroy | | yes | no | yes (Biosens-all BRET) | | https://www.domaintherapeutics.com/technologies/ |
| Fluorescence Size Exclusion Chromatography | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Genetically-Encoded Fluorescent GPCR Sensors | Tommaso | Patriarchi | Switzerland | yes | no | no | | https://www.patriarchilab.com/ |
| Immunofluorescence | Andreja | Ambriović-Ristov | Croatia | yes | yes | no | | |
| Immunoprecipitation | Zafiroula | Georgoussi | Greece | yes | yes | no | | |
| Immuno-Oncology Platform | Xavier | Leroy | | yes | no | yes (Biosens-all BRET) | | https://www.domaintherapeutics.com/technologies/ |
| In vitro enzymatic reactions | José | Zugaza | Spain | yes | yes | no | | |
| Isolation of Integrin Adhesion Complexes | Andreja | Ambriović-Ristov | Croatia | yes | yes | no | | |
| Label-Free technologies | Rafael | Franco | | yes | yes | no | | |
| Ligand Binding Assays | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| Ligand Binding Assays | Gebhard | Schertler | Switzerland | yes | yes | yes (Electron Microscopy; GPCR Structure; Crystallography) | | https://www.psi.ch/en/science; https://www.psi.ch/en/bio/schertler-group-research |
| Ligand Binding Assays | David | Sykes | UK | yes | yes | yes (thermostability based drug screening) | | https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1 |
| Monoclonal Antibodies | Xavier | Leroy | | yes | no | yes (Biosens-all BRET) | | https://www.domaintherapeutics.com/technologies/ |
| Protein Chemical Ligation | Juan Bautista | Blanco-Canosa | Spain | yes | yes | no | | |
| Protein Production and Purification | Martin | Caffrey | Ireland | yes | yes | no | | https://www.jove.com/t/1712/crystallizing-membrane-proteins-for-structure-determination-using |
| Recombinant Peptides | José | Zugaza | Spain | yes | yes | no | | |
| Solid Phase Peptide Synthesis (SPPS) | Juan Bautista | Blanco-Canosa | Spain | yes | yes | no | | |
| Thermostability Assays | David | Sykes | UK | yes | yes | yes (thermostability based drug screening) | | https://www.biorxiv.org/content/10.1101/2020.07.07.191957v1 |
| Thermophoresis | Isabel | Alves | France | yes | yes | no | | https://pubs.acs.org/doi/full/10.1021/acs.accounts.9b00007 |
Cell biology | | | | | | | | | |
| Apoptosis Assays | Nuno | Saraiva | Portugal | yes | no | no | | |
| Cell-based Assays | Beatriz | Bueschbell | Portugal | yes | yes | no | | |
| Cell Motility (Invasion/Migration; 2D/3D) | Nuno | Saraiva | Portugal | yes | no | no | | |
| CRISPR/Cas9 Cell Lines | Julien | Hanson | Belgium | yes | yes | no | | |
| Electrophysiology | Antonios | Kolocouris | Greece | yes | yes | no | | |
| GPCR heterologous expression and characterization in Xenopus oocytes | Christophe | Moreau | France | yes | yes | no | | |
| GPCR internalization | Shanna | Bastiaan-Net | Netherlands | yes | yes | yes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation) | | |
| Incucyte | Markus | Muttenthaler | Austria | yes | yes | yes (Custom peptide synthesis and probe development) | | |
| Insect cell culture | Andy | Chevigné | Luxembourg | yes | yes | yes (Drug screening, molecular profiling) | | |
| Mitochondria Assays | Silvana | Andric | Serbia | yes | yes | yes (In vivo models (rat, mice); molecular biology and functional assays of endocrine cells) | | |
| Reporter Cell Lines | Shanna | Bastiaan-Net | Netherlands | yes | yes | yes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation) | | |
Molecular biology | | | | | | | | | |
| CRISPR/Cas9 | Julien | Hanson | Belgium | yes | yes | no | | |
| CRISPR/Cas10 | Josef | Lazar | Czech Republic | yes | yes | no | | https://www.nature.com/articles/s42003-021-01694-1; https://www.nature.com/nmeth/journal/v8/n8/full/nmeth.1643.html https://www.pnas.org/content/pnas/117/51/32395.full.pdf; |
| IP-One Assay | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Mutagenesis, Chimeras, Phusions | Silvana | Andric | Serbia | yes | yes | yes (In vivo models (rat, mice); molecular biology and functional assays of endocrine cells) | | |
| Mutagenesis, Chimeras, Phusions | Rafael | Franco | | yes | yes | no | | |
| Mutagenesis, Chimeras, Phusions | Julien | Hanson | Belgium | yes | yes | no | | |
| Mutagenesis, Chimeras, Phusions | Christophe | Moreau | France | yes | yes | no | | |
| Pyrosequencing | Mauro | Maccarrone | Italy | yes | yes | no | | |
| qPCR | Shanna | Bastiaan-Net | Netherlands | yes | yes | yes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation) | | |
| Receptors in Baculoviruses | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| RNAseq | Shanna | Bastiaan-Net | Netherlands | yes | yes | yes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation) | | |
Pharmacology | | | | | | | | | |
| Ca 2+ Concentration Assays | Shanna | Bastiaan-Net | Netherlands | yes | yes | yes (Intestine-related research: cell culture, transcriptomics, calcium influx, receptor internalisation) | | |
| Ca 2+ Concentration Assays | Thierry | Capiod | France | yes | yes | yes (High-throughput drug screening (FDSSµCell)) | | |
| Ca 2+ Concentration Assays | Julien | Hanson | Belgium | yes | yes | no | | |
| cAMP Concentration Assays | Rafael | Franco | | yes | yes | no | | |
| cAMP Concentration Assays | Zafiroula | Georgoussi | Greece | yes | yes | no | | |
| cAMP Concentration Assays | Julien | Hanson | Belgium | yes | yes | no | | |
| cAMP Concentration Assays | Ago | Rinken | Estonia | yes | yes | yes (Ligand binding assay based on fluorescence anisotropy, preparation of cover glass and sample holders for ultrasensitive TIRF) | | DOI: 10.1039/d0nr06737g |
| ERK Phoshorylation | Rafael | Franco | | yes | yes | no | | |
| ERK Phoshorylation | Julien | Hanson | Belgium | yes | yes | no | | |
| Functional Analysis of Endocriine Cells | Silvana | Andric | Serbia | yes | yes | yes (In vivo models (rat, mice); molecular biology and functional assays of endocrine cells) | | |
| GTPase Assays | Mickey | Kosloff | Israel | yes | yes | no | | http://koslofflab.haifa.ac.il/papers.html |
| Intracellular ROS detection | Nuno | Saraiva | Portugal | yes | no | no | | |
| NanoLuc Complementation | Julien | Hanson | Belgium | yes | yes | no | | |
| Photopharmacological assays | Xavier | Rovira | Spain | yes | yes | yes (Drug design) | | https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037 |
| Protein-Protein Interactions (PPIs) Assays | Zafiroula | Georgoussi | Greece | yes | yes | no | | |
| Protein-Protein Interactions (PPIs) Assays | Mickey | Kosloff | Israel | yes | yes | no | | http://koslofflab.haifa.ac.il/papers.html |
| RhoGEF Activity | Julien | Hanson | Belgium | yes | yes | no | | |
| TGFalpha Shedding | Julien | Hanson | Belgium | yes | yes | no | | |
| | | | | | | | | |
Chemistry | | | | | | | | | |
| Combinatorial Synthesis (Conjugation and Bioconjugation) | Maria | Majellaro | Spain | yes | yes | yes (on-demand synthesis) | | https://www.celtarys.com |
| Enantioselective Synthesis/Chiral HPLC | Eddy | Sotelo | Spain | yes | yes | yes (custom synthesis and optimization of fluorescent ligands) | | |
| Enantioselective Synthesis/Chiral HPLC | Maria | Majellaro | Spain | yes | yes | yes (on-demand synthesis) | | https://www.celtarys.com |
| Fluorescent Ligand Development | Maria | Majellaro | Spain | yes | yes | yes (on-demand synthesis) | | https://www.celtarys.com |
| Fluorescent Ligand Development | Eddy | Sotelo | Spain | yes | yes | yes (custom synthesis and optimization of fluorescent ligands) | | |
| High-throughput screening of fluorescent ligands | Eddy | Sotelo | Spain | yes | yes | yes (custom synthesis and optimization of fluorescent ligands) | | |
| High-throughput drug screening (FDSSµCell) | Thierry | Capiod | France | yes | yes | yes (High-throughput drug screening (FDSSµCell)) | | |
| Hit to Lead Optimization | Marcel | Bermudez | Germany | yes | yes | no | | https://doi.org/10.1074/jbc.M116.735431, https://doi.org/10.1002/wcms.1468 |
| Hit to Lead Optimization | Maria | Majellaro | Spain | yes | yes | yes (on-demand synthesis) | | https://www.celtarys.com |
| Ligand-Based Drug Design | Katarina | Nikolic | Bulgaria | yes | yes | yes (deciphering off-targets of GPCR ligands) | | https://doi.org/10.1002/minf.201900165 |
| Medicinal Chemistry | Ester | Kellenberger | France | yes | yes | no | | |
| Medicinal Chemistry | Antonios | Kolocouris | Greece | yes | yes | no | | |
| Medicinal Chemistry | Markus | Muttenthaler | Austria | yes | yes | yes (Custom peptide synthesis and probe development) | | |
| Medicinal Chemistry | Holger | Stark | Germany | yes | yes | yes (binding assays dopamine and histamine receptor subtypes; MAO A/B screening; ligand synthesis) | | |
| Multivalent Ligand Design | Markus | Muttenthaler | Austria | yes | yes | yes (Custom peptide synthesis and probe development) | | |
| Nanoparticles | Frank | Bernhard | Germany | yes | yes | no | | Rues et al (2016) BBA 1858, 1306; Rues et al (2018) Methods 147, 73; Dong et al (2018) JMB 430, 5105; Krug et al (2020) Angew Chemie 59, 2. |
| Nuclear Magnetic Resonance (NMR) for compounds characterization | Eddy | Sotelo | Spain | yes | yes | yes (custom synthesis and optimization of fluorescent ligands) | | |
| Organic Compound Purification and Characterization | Cristina | Nativi | Italy | yes | yes | no | | |
| Organic Synthesis | Salvatore | Guccione | Italy | yes | yes | yes (Virtual Screening, Repositioning) | | |
| Organic Synthesis | Cristina | Nativi | Italy | yes | yes | no | | |
| Structure-based Drug Development | Anna Maria | Almerico | Italy | yes | yes | yes (in silico analysis and drug design) | | |
| Structure-based Drug Development | Fabian | Liessmann | Germany | yes | yes | no | | |
| Structure-based Drug Development | Katarina | Nikolic | Bulgaria | yes | yes | yes (deciphering off-targets of GPCR ligands) | | https://doi.org/10.1002/minf.201900165 |
| Structure-based Drug Development | Xavier | Rovira | Spain | yes | yes | yes (Drug design) | | https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037 |
| Structure-based Drug Development | Holger | Stark | Germany | yes | yes | yes (binding assays dopamine and histamine receptor subtypes; MAO A/B screening; ligand synthesis) | | |
| Synthesis of drugs with heterocyclic moieties | Anna Maria | Almerico | Italy | yes | yes | yes (in silico analysis and drug design) | | |
| Tracer Development | Markus | Muttenthaler | Austria | yes | yes | yes (Custom peptide synthesis and probe development) | | |
Animal models | | | | | | | | | |
| CRISPR/Cas9 Mice | Julien | Hanson | Belgium | yes | yes | no | | |
| Heart Failure with Preserved Ejection Fraction (HFpEF) Animal Model | Kjetil | Andressen | Norway | yes | yes | no | | |
| In vivo Imaging (Fibre Photometry) | Tommaso | Patriarchi | Switzerland | yes | no | no | | https://www.patriarchilab.com/ |
| Zebrafish Behhavioral Assays | Xavier | Rovira | Spain | yes | yes | yes (Drug design) | | https://www.iqac.csic.es/mcs/research/photopharmacology/; https://pubs.acs.org/doi/10.1021/acs.chemrev.8b00037 |
| | | | | | | | | |
Behavioral | | | | | | | | | |
| Online Surveys | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
| Sensory Evaluation in Human Panels (Taste Related) | Masha | Niv | Israel | yes | yes | no | | https://pubmed.ncbi.nlm.nih.gov/32807908/ |
Clinical | | | | | | | | | |
| Electrocardiography (ECG) | Belgin | Buyukakilli | Turkey | yes | no | no | | |
| Electromyopraphy (EMG) | Belgin | Buyukakilli | Turkey | yes | no | no | | |
| Electroneurography (ENG) | Belgin | Buyukakilli | Turkey | yes | no | no | | |
| Impedance Cardiography (ICG-TEB) | Belgin | Buyukakilli | Turkey | yes | no | no | | |
| | | | | | | | | |