WG1 + WG2 survey – methodologies

Available techniques and methodologies in respect to protein-protein interactions:Available techniques and methodologies in respect to cell physiology:Available resources in respect to cell physiology:If applicable, please specify, which type of pDNA/viral vectors, fluorescent biosensors, antibodies, etc.:Last name:First name:Contact e-mail:Institution/University:Country:              
X-ray crystallography;FRET techniques;BRET techniques;Mass spectrometry (MS)FRET imaging;BRET imaging;Confocal microscopy;Immunocytochemistry/Immunohistochemistry;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometry;Electrophysiology;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Fluorescent biosensors;Antibodies;Ligands;Transgenic cell lines;Animal (disease) models;Genomic/Transcriptomic data;LibrariesAbboudDayanaUniversity of LiegeBelgium
Bioinformatics;Molecular dynamics simulationsAcherFrancineUniversity of Paris / CNRSFrance
reproductive physiologyCell/tissue culture facilities;Animal research facilities;Animal (disease) models;Human/Animal tissue bankAndricSilvanaUniversity of Novi Sad Faculty of SciencesSerbia
Animal behavior studies;ex-vivo culturesCell/tissue culture facilities;Animal research facilities;Antibodies;Ligands;Animal (disease) models;Human/Animal tissue bankArdevolAnnaUniversitat Rovira i VirgiliSpain
Immunocytochemistry/Immunohistochemistry;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometryCell/tissue culture facilities;pDNA/Viral vectors;Transgenic cell linesToo many to list here.Bastiaan-NetShannaWageningen UR; Food & Biobased ResearchNetherlands
FRET techniques;BRET techniquesFRET imaging;BRET imagingCell/tissue culture facilities;Fluorescent biosensors;Transgenic cell linesVarious sensors for PPI and second messenger production based on NanoBiT, BRET and FRET, CRISPR/Cas 9 genome edited HEK293 cell lines lacking different effector proteins downstream of GPCRsBenkelTobiasUniversity of BonnGermany
FRET techniques;BRET techniquesCell/tissue culture facilities;Fluorescent biosensors;Ligands;Human/Animal iPSCH187 via MTA, Gq sensor via MTABiebermannHeikeCharité University medicine BerlinGermany
FRET techniques;BRET techniques;Bioinformatics;Molecular dynamics simulationsFRET imaging;BRET imaging;Fluorescence flow cytometry;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Antibodies;LigandsAll vectors used for BRET, FRET ans Shedding assayBirgulNeclaUniversity of BogaziciTurkey
NMR;Mass spectrometry (MS)Fluorimetry/Fluorimetric assaysCell/tissue culture facilities;Animal research facilitiesBlanco-CanosaJuan BautistaInstitute for Advanced Chemistry of Catalonia (IQAC-CSIC)Spain
FRET techniques;BRET techniques;Mass spectrometry (MS)FRET imaging;BRET imaging;Confocal microscopy;Immunocytochemistry/Immunohistochemistry;Fluorescence flow cytometry;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Fluorescent biosensors;Antibodies;Nanobodies;Ligands;Transgenic cell linesBozonVeroniqueINRAe and University of ToursFrance
X-ray crystallography;NMR;Cryo-electron microscopy;Mass spectrometry (MS)LigandsCatoireLaurentCNRS/University of ParisFrance
Molecular dynamics simulationsChabbertMarieCNRSFrance
Immunoprecipitation, GST fusion peptides encompassing various regions of opioid receptors as well as interactive partners spinophilin, STAT5BTwo (multi)-photon microscopy;Confocal microscopy;Immunocytochemistry/Immunohistochemistry;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometry;Electrophysiology;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Antibodies;Ligands;Transgenic cell linesStable cell lines expressing each of the opioid receptors, Various opioid ligands, SerotoninGeorgoussiZafiroula (Iro)Institute of Biosciences and Applications/National Centre for Scientific Research "Demokritos"Greece
Mass spectrometry (MS)Confocal microscopy;Mass spectrometry imaging;Immunocytochemistry/Immunohistochemistry;Fluorescence flow cytometryCell/tissue culture facilities;Ligands;Animal (disease) models;Genomic/Transcriptomic dataMany ligandsGruberChristianMedical University of ViennaAustria
Bioinformatics;Molecular dynamics simulationsHuman/Animal tissue bank;Genomic/Transcriptomic dataHauserAlexanderUniversity of DenmarkDenmark
Fluorescence flow cytometryCell/tissue culture facilities;LibrariesHoxhaMalvinaCatholic University Our Lady of Good CounselAlbania
BRET techniquesFRET imaging;Confocal microscopy;Fluorescence flow cytometryCell/tissue culture facilities;Animal research facilities;Fluorescent biosensors;LigandsAT1 angiotensin receptor plasmidsHunyadyLaszloResearch Centre for Natural SciencesHungary
FRET techniques;BRET techniquesFRET imaging;BRET imaging;Confocal microscopy;Fluorescence flow cytometryCell/tissue culture facilities;Fluorescent biosensors;Antibodies;Nanobodies;LigandsJean-AlphonseFredericCNRSFrance
Mass spectrometry (MS);BioinformaticsConfocal microscopy;Mass spectrometry imaging;Immunocytochemistry/Immunohistochemistry;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometry;Electrophysiology;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;pDNA/Viral vectors;Fluorescent biosensors;Antibodies;Animal (disease) modelsGFP-tagged GPR37KanBekiAcibadem UniversityTurkey
Bioinformatics;Molecular dynamics simulationsConfocal microscopy;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometryCell/tissue culture facilities;pDNA/Viral vectors;LigandsKinsellaGemmaTU DublinIreland
Bioinformatics;Molecular dynamics simulationsCell/tissue culture facilities;LigandsKolbPeterPhilipps-University MarburgGermany
Bioinformatics;Molecular dynamics simulationsLigands;LibrariesLigands for ARs, ligands for P2X7RsKolocourisAntoniosNational and Kapodistrian University of Athens, PharmacyGreece
FRET techniques;Super-resolution microscopy;BioinformaticsFRET imaging;Confocal microscopy;Fluorimetry/Fluorimetric assays;TIRF microscopyCell/tissue culture facilities;pDNA/Viral vectors;Fluorescent biosensors;LigandsBaculovirus vectors for the mentioned receptors, Epac cAMP biosensors, fluorescent ligands for muscarinic, dopaminergic and melanocortin 4 receptors.LaasfeldTonisUniversity of TartuEstonia
Bioinformatics;Molecular dynamics simulationsLatekDorotaUniversity of WarsawPoland
Bioinformatics;Molecular dynamics simulationsLigand LibrariesLiessmannFabianInstitute for Drug DiscoveryGermany
Bioinformatics;Molecular dynamics simulationsLouetMaximeUniversity of MontpellierFrance
FRET techniques;BRET techniquesFRET imaging;BRET imaging;Confocal microscopy;Mass spectrometry imaging;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometryCell/tissue culture facilities;pDNA/Viral vectors;Fluorescent biosensors;Antibodies;Nanobodies;Ligands;Transgenic cell lines;LibrariesManyMuellerChristaUniversity of BonnGermany
FRET techniques;BRET techniques;Super-resolution microscopyFRET imaging;BRET imaging;Super-resolution SIM microscopy;Confocal microscopy;Fluorescence flow cytometryCell/tissue culture facilities;pDNA/Viral vectors;Fluorescent biosensors;Antibodies;Nanobodies;Ligands;Transgenic cell linesFRET, BRET, single fluorophore biosensorsNemecKatarinaMDC BerlinGermany
Molecular dynamics simulationsLigandsOlgacAbdurrahmanGazi UniversityTurkey
Mass spectrometry (MS);Bioinformatics;Molecular dynamics simulationsConfocal microscopy; Immunocytochemistry/Immunohistochemistry; Fluorescence flow cytometry;Electrophysiology;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Fluorescent biosensors;Antibodies;Animal (disease) modelsGFP-tagged GPR37 plasmid, mitochondia spesific fluorescent probes, Autophagy proteins...Oz-ArslanDevrimAcibadem UniversityTurkey
Two (multi)-photon microscopy;Electrophysiology;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Fluorescent biosensorsParnasMosheTel Aviv UniversityIsrael
BRET techniquesBRET imagingCell/tissue culture facilities;pDNA/Viral vectors;Fluorescent biosensors;Antibodies;Nanobodies;LigandsPfeilEvaVrije Universiteit AmsterdamNetherlands
BRET techniques;Mass spectrometry (MS)Confocal microscopy; Immunocytochemistry/Immunohistochemistry; Fluorimetry/Fluorimetric assays;Fluorescence flow cytometryCell/tissue culture facilities;Animal research facilities;pDNA/Viral vectors;Fluorescent biosensors;Antibodies;Ligands;Transgenic cell lines;Animal (disease) models;Human/Animal tissue bank;Could have access to the others - in general have not used them.BRET biosensors, only commercially available ligandsRajagopalSudarshanDuke University School of MedicineUSA
FRET techniques;BRET techniques;Super-resolution microscopy;Molecular dynamics simulationsFRET imaging;BRET imaging;Super-resolution SIM microscopy;In vivo or live cell STED microscopy;Spinning disc microcopy;Fluorimetry/Fluorimetric assaysCell/tissue culture facilities;pDNA/Viral vectors;Fluorescent biosensors;Antibodies;Ligands;Transgenic cell linesWe use Bac-mam transfection in multibac system. It enables to transfect mammalian cells with multiple components with high efficacy. Systems are available for proteins/sensors listed aboveRinkenAgoUniversity of TartuEstonia
BRET techniquesBRET imaging;Confocal microscopy; Immunocytochemistry/Immunohistochemistry; Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Antibodies;Animal (disease) modelsScarpaMiriamUniversity of GlasgowUnited Kingdom
FRET techniques;BRET techniquesConfocal microscopy;Immunocytochemistry/Immunohistochemistry;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometryCell/tissue culture facilities;Animal research facilities;pDNA/Viral vectors;Antibodies;Nanobodies;Transgenic cell lines;Animal (disease) models;Genomic/Transcriptomic dataLentiviral vectors encoding CXCR4 and ACKR3 mutants - Human Papillomavirus genomes - many antibodiesSchlechtGeraldineUniversity Paris-SaclayFrance
Confocal microscopy;Fluorescence flow cytometryCell/tissue culture facilities;Animal research facilities;Antibodies;Transgenic cell linesThelenMarcusInstitute for Research in Biomedicine, Universita della Svizzera ItalianaSwitzerland
NMR;Cryo-electron microscopy;Mass spectrometry (MS)Confocal microscopy; Immunocytochemistry/Immunohistochemistry; Fluorescence flow cytometry;Seahorse XF for analyses of cell metabolism in real time.Cell/tissue culture facilities;Antibodies;Transgenic cell lines;Human/Animal iPSCWe use a wide range of antibodies to study ERK MAPK activity and molecular interactions within the ERK pathway and its crosstalk with other signaling pathways. We have developed transgenic cell lines that allow us to quantify ERK MAPK regulated transcription in the context of several tumor types.UldrijanStjepanMasaryk UniversityCzech Republic
BRET techniques;Bioinformatics;aequorin luminescence assay; reporter assaysBRET imaging;Confocal microscopy;Immunocytochemistry/Immunohistochemistry;Fluorimetry/Fluorimetric assays;Fluorescence flow cytometry;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Fluorescent biosensors;Ligands;Transgenic cell lines;Genomic/Transcriptomic dataVanden BroeckJozefKU LeuvenBelgium
X-ray crystallography;Super-resolution microscopy;Mass spectrometry (MS)Super-resolution SIM microscopy;Confocal microscopy;Fluorescence flow cytometryCell/tissue culture facilities;pDNA/Viral vectors;Antibodies;NanobodiesVanekOndrejCharles University, Faculty of ScienceCzech Republic
FRET imaging;Super-resolution SIM microscopy;Two (multi)-photon microscopy;Atomic force microscopy; Confocal microscopy; Immunocytochemistry/Immunohistochemistry; Fluorimetry/Fluorimetric assays;ElectrophysiologyCell/tissue culture facilities;Animal research facilities;pDNA/Viral vectors;Fluorescent biosensors;AntibodiesVardjanNinaUniversity of Ljubljana, Faculty of MedicineSlovenia
NMR;Super-resolution microscopy;Mass spectrometry (MS);Molecular dynamics simulationsIn vivo or live cell STED microscopy;Confocal microscopy;Mass spectrometry imaging; Immunocytochemistry/Immunohistochemistry; Fluorescence flow cytometry;Animal behavior studiesCell/tissue culture facilities;Animal research facilities;Ligands;Animal (disease) modelsVaversEdijsLatvian Institute of Organic SynthesisLatvia
Electron paramagnetic resonance (EPR) spectroscopy;FRET techniques;BRET techniquesConfocal microscopy; Immunocytochemistry/Immunohistochemistry; Electrophysiology;Animal behavior studiesCell/tissue culture facilities;Animal research facilitiesVreclMilkaUniversity of Ljubljana Slovenia
X-ray crystallography;Cryo-electron microscopy;BRET techniquesConfocal microscopy;Fluorescence flow cytometryCell/tissue culture facilities;Animal research facilities;Fluorescent biosensors;Antibodies;Nanobodies;LigandsWinglerLauraDuke UniversityUnited States
Super-resolution microscopy;Mass spectrometry (MS);BioinformaticsIn vivo or live cell STED microscopy;Atomic force microscopy;Confocal microscopy;Immunocytochemistry/Immunohistochemistry; Fluorimetry/Fluorimetric assays;Fluorescence flow cytometryCell/tissue culture facilities;Antibodies;microscopies, spectroscopiesZerovnikEvaJosef Stefan instituteSlovenia

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